Some Factors Influencing Respiration and Glycolysis in Ehrlich Ascites Tumor Cells*

The paper presents a study of various environmental factors which influence respira tion, glycolysis, and the Crabtree Effect in the Ehrlich ascites carcinoma cells. The factors examined for influence on respiration include: (1) pH in the presence and ab sence of glucose and (2) varying concentrations of inorganic phosphate in: (a) media used to wash the cells, (6) incubation media, with no supplementary substrate, with and without Tris buffer, (e) incubation medium containing 200 mg. per cent of DLlactate, (d) incubation media containing 55 mg. per cent of glucose and 37 HIMTris, (e) incubation medium containing only 0.5 mg. per cent glucose, (/) incubation medium containing 110 mg. per cent of 2-deoxyglucose, and (g) incubation media containing 110 mg. per cent of glucose, 37 HIMTris and 7 X 10~6M 2,4-dinitrophenol.